哪里有樣本釋放劑操作方法
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哪里有樣本釋放劑操作方法
廣州健侖生物科技有限公司
（廣州健侖生物科技有限公司是集研制開發(fā)、銷售、服務于一體的優(yōu)良企業(yè),公司產品涉及臨床快速診斷試劑、食品安全檢測試劑，違禁品快速檢測，動物疾病防疫檢測試劑，免疫診斷試劑、臨床血液學和體液學檢驗試劑、微生物檢驗試劑、分子生物學檢驗試劑、臨床生化試劑、有機試劑等眾多領域，同時核心代理Panbio、FOCUS、Qiagen、IBL、CORTEZ、Fuller、Inbios、BinaxNOW、LumuQuick、日本富士、日本生研等多家有名診斷產品集團公司產品，致力于為商檢單位、疾病預防控制中心、海關出入境檢疫局、衛(wèi)生防疫單位,緝毒系統(tǒng)，戒毒中心，檢驗檢疫單位、生化企業(yè)、科研院所、醫(yī)療機構等機構與行業(yè)提供*、高品質的產品服務。此外，本公司還開展食品、衛(wèi)生、環(huán)境、藥品等多方面的第三方檢測服務。）
哪里有樣本釋放劑操作方法
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【產品介紹】
二維碼掃一掃
【公司名稱】 廣州健侖生物科技有限公司
【】 楊永漢
【】
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-3室
【企業(yè)文化】
【Product Name】 Sample Release Agent
【Packing】 20 test / box (solution I: 20 × 1 Test / bottle; solution II: 20 Test / bottle)
50 test / box (solution I: 50x1 Test / bottle; solution II: 50 Test / bottle X1)
100 test / box (solution I: 100 x 1 Test / bottle; solution II: 50 Test / bottle x 2)
Detection method
(A) discharge antibody detection
Test tube method
(1) Label the test tube and add 1 drop of erythrocyte suspension of 2% known blood type, add 2 drops of the bleaching solution, mix and incubate at 37 ℃ for 30 minutes.
(2) Remove the test tube and wash the cells 3 times with saline. After the last wash, thoroughly decant the washing solution and blot the water in the test tube with absorbent paper.
(3) Add 2 drops of anti-human globulin reagent and mix well and centrifuge at 1000g for 15 seconds.
(4) gently shake the test tube, naked eye or under the microscope to read the results of agglutination.
2. Micro-column gel method
(1) Take anti-human globulin test card and make the mark, add 50μL of 0.8 ~ 1% red blood cell suspension of known blood type to the hole.
(2) Add 50 μL of the dispersion to the wells and incubate at 37 ° C for 15 minutes. Centrifuge using a card-type centrifuge.
(3) Remove the microcolumn gel card, the naked eye or interpreter read the agglutination results.
(B) detection of red cell antigen released after dissociation
1. To IgM monoclonal antibody detection of red blood cell antigen
(1) The dissociated red blood cells were washed 6 times with physiological saline, and then prepared into about 2% erythrocyte suspension with normal saline.
(2) Add 2 drops of antibody to a clean tube and label.
(3) Add 1 drop of 2% of the above suspension of erythrocytes to the test tube, gently mix and centrifuge at 1000g for 15 seconds.
(4) gently shake the test tube, naked eye or under the microscope to read the results of agglutination.
2. IgG monoclonal antibody detection of red blood cell antigen
(1) The dissociated red blood cells were washed 6 times with physiological saline, and then prepared into about 2% erythrocyte suspension with normal saline.
(2) Add 2 drops of antibody to a clean tube and label.
(3) Add 1 drop of 2% of the suspension of erythrocytes to be tested to the test tube and mix well and incubate in a water bath at 37 ° C. for 30 minutes.
(4) Remove the test tube and wash the cells three times with saline. After the last wash, thoroughly decant the washing solution and blot the water in the test tube with absorbent paper.
(5) Add 2 drops of anti-human globulin reagent and mix well and centrifuge at 1000g for 15 seconds.
(6) Gently shake the tube and read the agglutination results either naked or under the microscope.
【Reference】 This product is qualitative reagent, do not need to set the reference value.
[Interpretation of test results]
1. Positive reaction: the discharge and the corresponding erythrocytes in the test tube or agglutination of the corresponding agglutination of the distribution of the red blood cells in the gel surface or gel, indicating that the red blood cells have been sensitized by the antibody, the antibody solution.
2. Negative reactions: the discharge solution and the corresponding red blood cells in the test tube does not appear agglutination or the corresponding deposition of all red blood cells in the bottom of the gel tube, indicating that the red blood cells were not sensitized by the antibody, the discharge solution does not contain antibodies.
Limitations of test methods
1. Diluent can not be used to identify Duffy blood group antibody.
2. The erythrocytes treated by this kit can not be used for the phenotype detection of Kell's blood group system.
【Product Performance Index】
1. pH value (25 ° C ± 1 ° C): 1.7 ± 0.2 (Solution I) 9.0 ± 0.2 (Solution II)
2. Osmolarity: 450 ± 20 mOsm / L (Solution I) 130 ± 20 mOsm / L (Solution II)
【Precautions】
1. Diluent and the last wash cell supernatant to do parallel testing to confirm the serum free antibody has been compley removed.
2. Dissolution liquid test tube method, if the negative reaction, you need to add human IgG anti-D sensitized erythrocytes to confirm the test to ensure the effectiveness of anti-human globulin reagents.
3. Dissolution liquid added to the solution II to adjust the pH to neutral prone to sediment turbidity can be centrifuged to remove the precipitate after supernatant antibody detection.
