EB病毒早期抗原IgG免疫熒光玻片試劑盒

EBV Early Antigens IgG IFA Kit

廣州健侖生物科技有限公司

主要用途：用于檢測人血清中的EB病毒早期抗原IgG抗體

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EB病毒早期抗原IgG免疫熒光玻片試劑盒

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【公司名稱】 廣州健侖生物科技有限公司
【】    楊永漢 
【】 
【騰訊 】 2042552662
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號二期2幢101-3室
【企業(yè)文化】

起初，我們沒有獲得多大的成功，但在2005年，我們在技術(shù)上取得了突破。以前，我們實驗室在培養(yǎng)干細胞時，細胞只能平鋪在培養(yǎng)皿上，但在2005年，我們突破了“二維限制”，可以讓干細胞懸浮在培養(yǎng)液中，這就是“懸浮培養(yǎng)”。我們采用這種三維培養(yǎng)技術(shù)的原因有很多。首先，在懸浮培養(yǎng)中，細胞聚集時，本身就會形成三維結(jié)構(gòu)，因此在產(chǎn)生復(fù)雜組織時，會比平鋪的細胞層更容易;其次，為了發(fā)育成復(fù)雜的結(jié)構(gòu)，細胞之間需要相互交流，而三維培養(yǎng)更適于促進這樣的交流，因為細胞之間可以更加靈活地發(fā)生相互作用。
使用這種新方法，我們把相互分離的胚胎干細胞懸浮在液體培養(yǎng)基中，然后注入多孔培養(yǎng)皿的小孔中(每個小孔只有微量的培養(yǎng)基，含大約3 000個胚胎干細胞)。我們發(fā)現(xiàn)，原本分開的胚胎干細胞開始聚集在一起。
隨后，就可以誘導(dǎo)這些微小的細胞聚集體，讓它們?nèi)糠只癁橐环N神經(jīng)前體細胞(neural progenitor)——這類細胞通常存在于大腦前部。然后，這些細胞開始相互發(fā)送信號，經(jīng)過三四天的時間，它們便自發(fā)組織成中空的球體，由單層的神經(jīng)上皮細胞構(gòu)成(神經(jīng)上皮細胞即神經(jīng)干細胞，由前體細胞分化而來)。我們把這種形成單細胞層的方法稱作SFEBq培養(yǎng)法，即“胚狀體樣聚集體的快速再聚集無血清懸浮培養(yǎng)法”(serum-free floating culture of embryoid body-like aggregate with quick reaggregation)。
在胚胎中，神經(jīng)上皮細胞接收來自細胞外的化學(xué)信號，zui終形成特異的大腦組織結(jié)構(gòu)。在這些化學(xué)信號中，有一種信號可觸發(fā)間腦的發(fā)育，形成視網(wǎng)膜和下丘腦(hypothalamus，控制食欲和其他許多基本生理功能的腦區(qū))。成功地使胚胎干細胞形成球體之后，我們實驗室開始嘗試，誘導(dǎo)這些細胞分化成視網(wǎng)膜前體細胞——成熟視網(wǎng)膜細胞的前體。我們向SFEBq培養(yǎng)體系中加入了一系列蛋白質(zhì)。在胚胎中，這些蛋白的作用正是誘導(dǎo)視網(wǎng)膜前體細胞的產(chǎn)生。

At first, we did not get much success, but in 2005, we made a technological breakthrough. In the past, when we cultured stem cells in our laboratory, the cells were only laid on the culture dish. However, in 2005, we broke through the "two-dimensional limitation" and allowed the suspension of stem cells in the culture medium. This is called "suspension culture." There are many reasons why we use this three-dimensional culture technique. First, in suspension culture, cells accumulate and form three-dimensional structures themselves, making them easier to produce complex tissues than tiled cell layers. Second, cells need to communicate with each other in order to develop complex structures , While three-dimensional c*tion is better suited to facilitate such exchanges because the cells can interact more flexibly.
Using this new method, we suspended the embryonic stem cells isolated from each other in liquid medium and injected them into the wells of a multi-well culture dish (with only a minimal amount of media per well containing approximay 3,000 embryonic stem cells). We found that originally separated embryonic stem cells began to congregate.
Subsequently, these tiny cell aggregates can be induced to differentiate them all into a neural progenitor - usually in the front of the brain. The cells then started to send signals to each other. After three or four days, they spontaneously organized into hollow spheres and consisted of a single layer of neuroepithelial cells (neural epithelial cells, neural stem cells, differentiated from precursor cells). We refer to this method of forming a single cell layer as the SFEBq culture method, that is, "serum-free floating culture of embryoid body-like aggregate with quick reaggregation" .
In embryos, neuroepithelial cells receive chemical signals from outside the cell, eventually forming specific brain tissue structures. Among these chemical signals, there is a signal that triggers the development of the diencephalon that forms the retina and the hypothalamus (the brain that controls appetite and many other basic physiological functions). After successfully bringing embryonic stem cells into the sphere, our lab started trying to induce these cells to differentiate into precursors of retinal precursor cells, mature retinal cells. We added a series of proteins to the SFEBq system. In embryos, the function of these proteins is to induce the production of retinal progenitor cells.